For investigate functions, RT‐qPCR is usually used not merely to determine the presence or absence of an RNA but to measure its concentration. In cases like this, a calibration curve need to be included in each and every experiment, consisting of no less than six serial dilutions of the in vitro−transcribed RNA typical. The starting up focus an
For exploration purposes, RT‐qPCR is usually used not merely to find out the existence or absence of an RNA but to measure its focus. In cases like this, a calibration curve should be included in every experiment, consisting of at the very least six serial dilutions of the in vitro−transcribed RNA normal. The setting up concentration and fold d
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